Nucleic acid enzymology of extremely halophilic bacteria. Halobacterium cutirubrum polynucleotide phosphorylase
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چکیده
منابع مشابه
Studies of the electron transport chain of extremely halophilic bacteria. I. Spectrophotometric identification of the cytochromes of Halobacterium cutirubrum.
A scheme of electron transport in Halobacterium cutirubrum is proposed based on the following findings. (a) The slow rate of reduction of b-type cytochromes by DPNH is not consistent with the participation of these components in the main pathway of the oxidation of DPNH. (b) DPNH oxidation proceeds through a fast, 2-heptyl-4-hydroxyquinoline N-oxide (QO)-sensitive and a slow, QO-insensitive pat...
متن کاملUnusual evolution of a superoxide dismutase-like gene from the extremely halophilic archaebacterium Halobacterium cutirubrum.
The archaebacterium Halobacterium cutirubrum contains a single detectable, Mn-containing superoxide dismutase, which is encoded by the sod gene (B. P. May and P. P. Dennis, J. Biol. Chem. 264:12253-12258, 1989). The genome of H. cutirubrum also contains a closely related sod-like gene (slg) of unknown function that has a pattern of expression different from that of sod. The four amino acid resi...
متن کاملProteolytic enzymes from extremely halophilic bacteria.
Halobacterium strains produce a truly extracellular proteinase which degrades gelatine and casein. It has a pH optimum of about 8 and depends upon divalent cations and a high concentration of NaCl or KC1 for activity and stability. Proteolytic enzymes were also found in cell homogenates obtained by ultra sonic treatment. A caseinolytic enzyme, probably different from the extracellular one, is a...
متن کاملGel-filtration and Density-gradient-centrifugation Studies of the Molecular Weights of Halobacterium Cutirubrum
1. Conditions have been established for the estimation of molecular weights of proteins by analytical gel filtration and sucrose-density-gradient centrifugation in 2.5M-potassium chloride-l M-sodium chloride; Halobacterium cutirubrum polynucleotide phosphorylase, DNA-dependent RNA polymerase and RNA-dependent RNA polymerase have been studied by these methods. 2. The RNA-dependent polymerase has...
متن کاملPurification and properties of the ribonucleic acid-dependent ribonucleic acid polymerase from Halobacterium cutirubrum.
1. The RNA-dependent RNA polymerase from Halobacterium cutirubrum was purified to electrophoretic homogeneity. 2. It requires a single-stranded molecule of RNA or polyribonucleotide as template. 3. Nearest-neighbour analyses of the products formed on random poly(A,U) or alternating poly(A-U) templates and base analysis of the product of synthesis directed by wheat-germ RNA prove that the templa...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1971
ISSN: 0306-3283
DOI: 10.1042/bj1210613